Multi-Plane Microscopy (also called multi-foci microscopy) is a relatively recent 3D imaging modality. Originally created by adding a beam splitter after the tube lens to generate two images on the camera which, by tuning the optical length between the tube lens and the camera for each path (one slightly longer resp. shorter than the other) creates two images at two different depths in the sample both in focus at the camera. At Hofkens lab, we have a more advanced version in which the beam splitter was replaced by a prism (see Fig. below) which split the light after the tube lens in eight images while tuning the distance of individual beam of light such that they lead images at different depth in the sample. By doing so we can image simultaneously a 50x50x4 µm volume.